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KMID : 0545119960060010043
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 1 p.43 ~ p.49
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Modulation of Phosphoenolpyruvate Metabolism of Anaerobiospirillum succiniciproducens ATCC 29305
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YOO JIN-YOUNG
J. GREGORY ZEIKUS
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Abstract
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Modulation of the catabolic PEP-pathway of Anaerobiospirillum succiniciproducens was tried using some enzymatic inhibitors such as gases and chemicals in order to enhance succinic acid production. 10% CO increased the succinic acid/acetic acid (S/A) ratio but inhibited growth as well as production of succinic and acetic acid. Hydrogen gas also increased the S/A ratio and inhibited the synthesis of pyruvate: ferredoxin oxidoreductase when used in mixture with CO_2,. Catabolic repression by acetic, lactic and formic acid was not recognized and other modulators such as glyoxylate, pyruvate derivatives, arsenic salt, phosphate and sulfate were shown not to be effective. Magesium carbonate was shown effective for repressing acetate production. Palmitic acid, myristic acid and phenylalanine did not affect acetate production but carprylic acid completely inhibited growth.
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KEYWORD
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